Studies on a nuclease from Penicillium citrinum. VI. Secondary structure of nuclease P1.
نویسندگان
چکیده
منابع مشابه
P1 nuclease defines a subpopulation of active SV40 chromatin - a new nuclease hypersensitivity assay
Under exhaustive digestion conditions P1 nuclease was found to cleave a subpopulation of intracellular SV40 chromatin only once. The major P1 cleavage site in SV40 DNA was mapped at the origin of DNA replication, and the two minor sites at the SV40 enhancers. The P1-sensitive SV40 chromatin subpopulation was found to have higher superhelical density than the bulk of the intracellular SV40 chrom...
متن کاملAdsorption of Nuclease P1 on Chitosan Nano-particles
The sorption of nuclease P1 onto chitosan nano-particles is studied in this paper. The effect of some adsorption kinetics factors such as nuclease P1 concentration, chitosan nano-particles solution concentration, adsorption temperature, chitosan nano-particles size, solution pH, etc. is investigated. Adsorption of nuclease P1 onto chitosan nano-particles is fitted into Lagergren first-order equ...
متن کاملImmobilization of Nuclease p1 on Chitosan Micro-spheres
L. E. Shi, Z. X. Tang, Y. Yi, J. S. Chen, W. Y. Xiong, and G. Q. Ying College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China Department of Food Science and Technology, College of Biotechnology, East China University of Science and Technology....
متن کاملScaled-down nuclease P1 for scaled-up DNA digestion.
Motivated by an interest in detecting DNA adducts in their nucleotide form by mass spectrometry (1), we sought efficient, low-cost conditions for enzymatic digestion of a relatively large amount of DNA (≥ 1 mg). Of the enzymes available for such digestion, nuclease P1, which forms deoxynucleoside-5′-monophosphates, seemed to be a good initial choice because it is widely used for digesting DNA, ...
متن کاملDraft Genome Sequence of Burkholderia sp. Strain PML1(12), an Ectomycorrhizosphere-Inhabiting Bacterium with Effective Mineral-Weathering Ability
We report the draft genome sequence of Burkholderia sp. PML1(12), a soil bacterium isolated from the Oak-Scleroderma citrinum ectomycorrhizosphere in the experimental forest site of Breuil-Chenue (France).
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1975
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.39.2145